GLICKMAN ANNOUNCES IMPROVEMENT IN TESTING FOR E. COLI O157:H7 Release No.0362.99 Andy Solomon (202) 720-4623 andy.solomon@usda.gov Carol Blake (202) 720-9113 carol.blake@usda.gov GLICKMAN ANNOUNCES IMPROVEMENT IN TESTING FOR E. COLI O157:H7 WASHINGTON, Sept. 10, 1999 -- Agriculture Secretary Dan Glickman today announced that USDA's Food Safety and Inspection Service (FSIS) has begun using a more sensitive method for detecting E. coli 0157:H7 in raw meat products. The new method is approximately four times more sensitive than previous methods, greatly increasing the probability of detecting very low levels of the potentially dangerous pathogen. "This is another significant step forward in our continuing efforts to protect consumers from foodborne illness," said Glickman. FSIS conducts E. coli O157:H7 tests on samples collected at federally- inspected plants that produce ground beef and at the approximately 100,000 retail outlets that grind beef on a regular basis. FSIS's testing program has also stimulated industry action to reduce the presence of the pathogen in ground beef. The new testing method, called "immunomagnetic separation" (IMS), will be incorporated into the standard procedures in use in all three FSIS field service laboratories. E. coli O157:H7 can cause serious illness or death when consumed in contaminated foods. Water and a variety of foods can harbor the pathogen. Because consumers may not cook meat thoroughly enough to kill pathogens that have been introduced below the surface, FSIS has declared that raw ground beef and other non-intact raw beef contaminated with E. coli O157:H7 is adulterated. FSIS advises consumers to use a thermometer and cook ground beef to an internal temperature of 160 degrees F. The IMS method begins after a screening test identifies a potentially positive sample. Antibody-coated magnetic beads recognize and capture E. coli O157:H7 cells in meat product enrichment broths. A magnetic device captures the beads after they bind to the pathogen. As a result, live E. coli O157:H7 cells are concentrated to facilitate growth and detection on special agar culture media. E. coli O157:H7 is then confirmed by standard FSIS procedures. The test will be used effective immediately in FSIS' Athens, Ga., laboratory and will be incorporated at the other FSIS laboratories in Alameda, Calif., and St. Louis, Mo., as soon as the necessary equipment is in place. Until all laboratories are properly equipped, all E. coli O157:H7 tests will be conducted at the Athens laboratory. #